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acrylamide bis crosslinking  (Bio-Rad)


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    Bio-Rad acrylamide bis crosslinking
    Acrylamide Bis Crosslinking, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 10173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acrylamide+bis+crosslinking/Acrylamide/10__22175_slash_mmb__15701-88-55-93
    Average 97 stars, based on 10173 article reviews
    acrylamide bis crosslinking - by Bioz Stars, 2026-09
    97/100 stars

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    Article Title: Title Pending 15701
    Article Snippet: Protein gel samples were thawed, and 9 μg protein/ lane was loaded onto 1.5-mm thick 15% polyacrylamide separating gels (0.38MTris, pH 8.8; 0.1% [wt/vol] SDS; 0.05% [wt/vol] ammonium persulfate [AMPER]; 0.05% [vol/vol] TEMED) with 5% polyacrylamide stacking gel (0.125 M Tris, pH 6.8; 0.1% [wt/vol] SDS; 0.075% [wt/vol] AMPER; 0.125% [vol/vol] TEMED) both with 37.5:1 acrylamide:bis crosslinking and run at a constant voltage of 120 V for 2 h 15 min in a running buffer (25 mM Tris; 0.192 M glycine; 2.0 mM EDTA; 0.1% [wt/vol] SDS) (Melody et al., 2004) on a BioRad Mini-PROTEAN Tetra Cell system (BioRad Laboratories, Hercules, CA).



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    Concentration dependence of spermine 4+ . (a) Chemical structure of spermine 4+ . (b) Diffusion coefficients D 1 of PAm-PAc gels with different c(spermine). (c) Fraction of the fast mode obtained by CONTIN fit. (d) c(spermine) dependence of zeta potential. PAm-PAc was polymerized without chemical crosslinker.

    Journal: bioRxiv

    Article Title: Microstructure evolution of multivalent ion-complexed polyelectrolyte gels

    doi: 10.1101/2025.11.10.687581

    Figure Lengend Snippet: Concentration dependence of spermine 4+ . (a) Chemical structure of spermine 4+ . (b) Diffusion coefficients D 1 of PAm-PAc gels with different c(spermine). (c) Fraction of the fast mode obtained by CONTIN fit. (d) c(spermine) dependence of zeta potential. PAm-PAc was polymerized without chemical crosslinker.

    Article Snippet: Crosslinker bis-acrylamide solution (MBAA) (2 wt%), calcium chloride (CaCl 2 ), magnesium chloride (MgCl 2 ), aluminum chloride (AlCl 3 ) hexahydrate, and barium chloride (BaCl 2 ) were purchased from Macklin.

    Techniques: Concentration Assay, Diffusion-based Assay, Zeta Potential Analyzer